Rapid Detection of the Three Celiac Disease Risk Genotypes HLA-DQ2.2, HLA-DQ2.5, and HLA-DQ8 by Multiplex Ligation-Dependent Probe Amplification
نویسندگان
چکیده
منابع مشابه
Rapid HLA typing by multiplex amplification refractory mutation system.
AIMS To detect HLA susceptibility and protective alleles associated with insulin dependent diabetes mellitus (IDDM) using a multiplex amplification refractory mutation system (ARMS). These include DR3 and DR4 alleles at the DRB1 locus, presence or absence of aspartic acid at position 57 (Asp-57) of the DQB1 locus, and presence or absence of arginine at position 52 (Arg-52) of the DQA1 locus. ...
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Salmonella enterica serovar Typhi, the causative agent of typhoid fever, is highly clonal and genetically conserved, making isolate subtyping difficult. We describe a standardized multiplex ligation-dependent probe amplification (MLPA) genotyping scheme targeting 11 key phylogenetic markers of the S. Typhi genome. The MLPA method demonstrated 90% concordance with single nucleotide polymorphism ...
متن کاملAnalysis of HLA and non-HLA alleles can identify individuals at high risk for celiac disease.
BACKGROUND & AIMS Celiac disease (CD) is a common chronic disorder of the small intestine, resulting from aberrant cellular responses to gluten peptides, and often remains undiagnosed. It is a complex genetic disorder, although 95% of the patients carry the risk heterodimer human leukocyte antigen (HLA)-DQ2. Genome-wide association studies on CD have identified 9 non-HLA loci that also contribu...
متن کاملApplications of multiplex ligation-dependent probe amplification (MLPA) method in diagnosis of cancer and genetic disorders
Introduction: Lots of human diseases and syndromes result from partial or complete gene deletions and duplications or changes of certain specific chromosomal sequences. Many various methods are used to study the chromosomal aberrations including Comparative Genomic Hybridization (CGH), Fluorescent in Situ Hybridization (FISH), Southern blots, Multiplex Amplifiable Probe Hybridisation (MAP...
متن کاملRisk of celiac disease according to HLA haplotype and country.
for the RIVUR Trial Investigators Since publication of their article, the authors report no further potential conflict of interest. 1. Hoberman A, Keren R. Antimicrobial prophylaxis for urinary tract infection in children. N Engl J Med 2009;361:1804-6. 2. Ingelfinger JR, Stapleton FB. Antibiotic prophylaxis for vesicoureteral reflux — answers, yet questions. N Engl J Med 2014;370:2440-1. 3. Nel...
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ژورنال
عنوان ژورنال: Genetic Testing and Molecular Biomarkers
سال: 2016
ISSN: 1945-0265,1945-0257
DOI: 10.1089/gtmb.2015.0233